期刊
BIO-PROTOCOL
卷 8, 期 1, 页码 -出版社
BIO-PROTOCOL
DOI: 10.21769/BioProtoc.2680
关键词
NPY; Serotonin; Exocytosis; Dense core vesicle (DCV); Neuroendocrine; High throughput screen; BON cells
类别
资金
- National Institutes of Health [DK040428, DK025861, GM119158]
Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay using DCV cargo, NPY-Venus, and an orthogonal H-3-serotonin secretion assay. The NPY-Venus secretion assay was successfully used for a high throughput siRNA screen, and the serotonin secretion assay was used to validate hits identified from the screen (Sorensen, 2017; Zhang et al., 2017).
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