期刊
SCIENCE
卷 358, 期 6363, 页码 672-676出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aan6516
关键词
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资金
- MIRA (Maximizing Investigators' Research Award) grant from the National Institutes of Health [R35GM118026]
- EMBL
- European Research Council (ERC) Consolidator Grant CondStruct [ERC-2015-CoG 681365]
- ERC Advanced Grant SynDiv [ERC-ADG-2014 669598]
- Netherlands Organization for Scientific Research (NWO/OCW) (as part of the Frontiers of Nanoscience program)
- Japan Society for the Promotion of Science
- Uehara Memorial Foundation
- EIPOD (EMBL Interdisciplinary Postdocs) fellowship under Marie Curie Actions (COFUND)
- European Molecular Biology Organization short-term fellowship
Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. We used single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of adenosine triphosphate hydrolysis-dependent translocation along double-stranded DNA. Condensin's translocation activity is rapid and highly processive, with individual complexes traveling an average distance of >= 10 kilobases at a velocity of similar to 60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its similar to 50-nanometer coiled-coil subunits, indicative of a translocation mechanism that is distinct from any reported for a DNA motor protein. The finding that condensin is a mechanochemical motor has important implications for understanding the mechanisms of chromosome organization and condensation.
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