期刊
SCIENCE
卷 355, 期 6327, 页码 836-841出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aah3605
关键词
-
资金
- Stanford Graduate Fellowship
- HHMI International Graduate Student Fellowship
- Stanford School of Engineering Undergraduate Visiting Researcher Program
- NIH Pioneer Award [5DP1GM111003]
Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light.
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