期刊
SCIENCE
卷 355, 期 6329, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf4831
关键词
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资金
- U.S. NSF [MCB-1026068, MCB-1158201]
- Natural Sciences and Engineering Research Council of Canada
- GenScript
- Cancer Center Support Grant at the Laura and Isaac Perlmutter Cancer Center [P30CA016087]
- University of Edinburgh
- Scottish Universities Life Sciences Alliance
- UK Biotechnology and Biological Sciences Research Council [BB/M005690/1, BB/M025640/1, BB/M00029X/1]
- BBSRC [BB/M00029X/1, BB/M025640/1, BB/M005690/1, BB/M005690/2] Funding Source: UKRI
- EPSRC [EP/P017401/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M005690/1, BB/M00029X/1, BB/M005690/2, BB/M025640/1, BBS/OS/GC/000001] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/P017401/1] Funding Source: researchfish
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1616111] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1445545] Funding Source: National Science Foundation
We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; Sc2.0 coding changes reduced Pre4 protein accumulation by half. Completing Sc2.0 specifies consolidation of 16 synthetic chromosomes into a single strain. We investigated phenotypic, transcriptional, and proteomewide consequences of Sc2.0 chromosome consolidation in poly-synthetic strains. Another bug was discovered through proteomic analysis, associated with alteration of the HIS2 transcription start due to transfer RNA deletion and loxPsym site insertion. Despite extensive genetic alterations across 6% of the genome, no major global changes were detected in the poly-synthetic strain omics analyses. This work sets the stage for completion of a designer, synthetic eukaryotic genome.
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