期刊
SCIENCE
卷 355, 期 6330, 页码 1211-1215出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf8451
关键词
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资金
- Swiss National Foundation (SNF) [31003053074, Sinergia CRSII_154414/1]
- Novartis FreeNovation program
- Swedish Research Council [2012-262: 2015-00635]
- SNF Ambizione fellowship [PZ00P3_136712]
- SNF Muse Heim-Voglin stipend
- ETH Excellence Scholarship and Opportunity Program stipend
Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.
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