期刊
SCIENCE
卷 355, 期 6329, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf3981
关键词
-
资金
- National Natural Science Foundation of China [31471254, 21621004, 21390203]
- Chinese Ministry of Science and Technology [2012CB725201, 2012AA02A708]
- Ph.D. Programs Foundation of Ministry of Education of China [20110002120055]
- Research Fund for the Doctoral Program of Higher Education of China [20120002110022]
- Tsinghua University Initiative grant [2011Z02296]
- NSF [MCB-1026068, MCB-1158201, MCB-1445545]
- BBSRC [BB/M005690/1] Funding Source: UKRI
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1445545] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/M005690/1] Funding Source: researchfish
We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in Saccharomyces cerevisiae. SynXII was assembled using a twostep method, specified by successive megachunk integration and meiotic recombinationmediated assembly, producing a functional chromosome in S. cerevisiae. Minor growth defect bugs detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a Saccharomyces synXII strain that would be identified as Saccharomyces bayanus by standard DNA barcoding procedures.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据