期刊
SCIENCE
卷 355, 期 6329, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.aaf4791
关键词
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资金
- China National High Technology Research and Development Program (863 Program) [2012AA02A708]
- National Natural Science Foundation of China [21621004, 21390203]
- University of Edinburgh
- Scottish Universities Life Sciences Alliance and Biotechnology and Biological Sciences Research Council [BB/M005690/1, BB/M025640/1, BB/M00029X/1]
- Wellcome Trust [090903, 092076]
- U.S. NSF [MCB-1026068, MCB-1158201, MCB-1445545]
- U.S. Department of Energy [DE-FG02097ER25308]
- National Science Foundation of China [31471254]
- Chinese Ministry of Science and Technology [2012CB725201]
- ERASynBio
- Agence Nationale pour la Recherche [IESY ANR-14-SYNB-0001-03]
- BBSRC [BB/M025640/1, BB/M005690/1, BB/M005690/2, BB/N016858/1, BB/K007211/1, BB/M00029X/1, BB/K007211/2] Funding Source: UKRI
- EPSRC [EP/P017401/1] Funding Source: UKRI
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1616111] Funding Source: National Science Foundation
- Biotechnology and Biological Sciences Research Council [BB/M00029X/1, BB/M005690/1, BB/M005690/2, BB/N016858/1, BB/M025640/1, BB/K007211/1, BBS/OS/GC/000001, BB/K007211/2] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/P017401/1] Funding Source: researchfish
Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37 degrees C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
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