期刊
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
卷 45, 期 2, 页码 146-154出版社
WILEY
DOI: 10.1111/1440-1681.12873
关键词
calcium sensitivity; inactivity; muscle fibre; prostate cancer; testosterone deprivation
资金
- National Health and Medical Research Council of Australia [1051460]
The contractile properties of vastus lateralis muscle fibres were examined in prostate cancer (PrCa) patients undergoing androgen deprivation therapy (ADT) and in age- and activity-matched healthy male subjects (Control). Mechanically-skinned muscle fibres were exposed to a sequence of heavily Ca2+-buffered solutions at progressively higher free [Ca2+] to determine their force-Ca2+ relationship. Ca2+-sensitivity was decreased in both type I and type II muscle fibres of ADT subjects relative to Controls (by -0.05 and -0.04 pCa units, respectively, P<.02), and specific force was around 13% lower in typeI fibres of ADT subjects than in Controls (P=.02), whereas there was no significant difference in type II fibres. Treatment with the reducing agent dithiothreitol slightly increased specific force in type I and type II fibres of ADT subjects (by similar to 2%-3%, P<.05) but not in Controls. Pure type IIx fibres were found frequently in muscle from ADT subjects but not in Controls, and the overall percentage of myosin heavy chain IIx in muscle samples was 2.5 times higher in ADT subjects (P<.01). The findings suggest that testosterone suppression can negatively impact the contractile properties by (i) reducing Ca2+-sensitivity in both type I and type II fibres and (ii) reducing maximum specific force in type I fibres.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据