期刊
SENSING AND BIO-SENSING RESEARCH
卷 17, 期 -, 页码 25-29出版社
ELSEVIER
DOI: 10.1016/j.sbsr.2018.01.003
关键词
Field effect transistor biosensor; Amyloid beta fibrils; Alzheimer's disease; Human serum albumin; Albumin-amyloid beta complexes
资金
- Center of Innovation Program, Building of Consortia for the Development of Human Resources in Science and Technology
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
As amyloid-beta peptide 1-42 (A beta 42) was found to be an emerging and important biomarker for Alzheimer's disease, the detection of this peptide in biological samples such as human serum (HS) has become very important for evaluating the potential disease state and determining the appropriate treatment. In this study, we developed an electrical analysis strategy based on a field effect transistor (FET) biosensor as a simple and reliable technique for confirming the presence of A beta 42 aggregates (fibrils) in biological samples. By utilizing Congo red immobilized on the FET gate surface as a biorecognition element, we observed remarkable sensitivity and specificity for detecting A beta 42 fibrils. Furthermore, we optimized the procedure to minimize the interference of abundant human serum albumin for the detection system using HS samples. The optimized system of Congo red-immobilized FET enables measurement of A beta 42 fibrils in the 100-pM level in HS samples, which is lower than its clinical concentration. The FET device can be applied as a biosensing system for mass and routine screening of peptide biomarkers related to Alzheimer's disease.
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