期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 82, 期 1, 页码 106-109出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2017.1403881
关键词
Microbial transglutaminase; enzymatic activity; thermostability; penta-site mutagenesis; Escherichia coli
类别
资金
- National High Technology Research and Development Program (863 Program) of China [2013AA102201]
- Major Project of Science and Technology of Anhui Province, China [1301031031]
- Fundamental Research Funds for the Central Universities, China [JZ2016HGBZ0777]
- Anhui Provincial Natural Science Foundation [JZ2017AKZR0123]
Microbial transglutaminase (MTG) is an enzyme widely used in the food industry. Mutiple-site mutagenesis of Streptomyces mobaraensis transglutaminase was performed in Escherichia coli. According to enzymatic assay and thermostability study, among three penta-site MTG mutants (DM01-03), DM01 exhibited the highest enzymatic activity of 55.7 +/- 1.4 U/mg and longest half-life at 50 degrees C (418.2min) and 60 degrees C (24.8min).
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