期刊
RUSSIAN JOURNAL OF GENETICS
卷 53, 期 3, 页码 314-324出版社
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1022795417030115
关键词
wheat-wheatgrass substituted cultivars; 6Ag(i) and 6Ag(i)2 chromosomes
资金
- RSF [16-16-00097]
- Leading Scientific Schools [NSh-8315.2016.11]
- [53.09]
- Russian Science Foundation [16-16-00097] Funding Source: Russian Science Foundation
A comparative study of wheat-wheatgrass substituted cultivars and lines resistant to leaf rust developed by the Agricultural Research Institute for Southeast Regions (Multi 6R, Belyanka, Favorit, Voevoda, Lebedushka) and Samara Agricultural Research Institute (Tulaikovskaya 5, Tulaikovskaya 10, Tulaikovskaya 100, Tulaikovskaya Zolotistaya) breeding was conducted. A complex analysis using molecular cytogenetic (C-differential banding, fluorescent (FISH) and genomic (GISH) in situ hybridization), molecular (PLUG markers), and biochemical (electrophoretic analysis of gliadins) markers demonstrated that they have a substitition of wheat chromosome 6D by the chromosomes 6Ag(i) and 6Ag(i)2 belonging to the J(=E) Agropyron intermedium (Host) Beauv (=Thinopyrum intermedium (Host) Barkworth & D.R. Dewey) subgenome. In spite of the fact that the chromosomes 6Ag(i) and 6Ag(i)2 differ in the C-banding pattern and demonstrated minor differences in the blocks of gliadin components, they had the identical pattern of pSc119.2 and pAs1 probe distribution and conjugated between themselves with insignificant disturbance. Thus, it was demonstrated that 6Agi and 6Ag(i)2 are homologous chromosomes; however, the question about allelism of their leaf rust resistance genes between themselves requires special studies. Nevertheless, using STS and SCAR markers and taking into account the type of reaction to Puccinia triticina, their non-allelism to the Lr9, Lr19, Lr24, Lr29, Lr38, and Lr47 genes was established. It was revealed that the 6Ag(i) and 6Ag(i)2 chromosomes have a different level of transmission in hybrid F-2 populations depending on the hybrid combination gene background.
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