期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 65, 期 1, 页码 62-68出版社
WILEY
DOI: 10.1002/bab.1577
关键词
size-exclusion chromatography; small-angle X-ray scattering; hydrogen; deuterium exchange mass spectrometry; RTX motif; calcium
资金
- CNRS
- Institut Pasteur
- program PasteurInnoV
- CACSICE
Small-angle X-ray scattering (SAXS) is a relatively simple experimental technique that provides information on the global conformation of macromolecules in solution, be they fully structured, partially, or extensively unfolded. Size exclusion chromatography in line with a SAXS measuring cell considerably improves the monodispersity and ideality of solutions, the two main requirements of a good SAXS sample. Hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) offers a wealth of information regarding the solvent accessibility at the local (peptide) level. It constitutes a sensitive probe of local flexibility and, more generally, of structural dynamics. The combination of both approaches presented here is very powerful, as illustrated by the case of RD, a calcium-binding protein that is part of a bacterial virulence factor.
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