期刊
ANDROLOGIA
卷 50, 期 2, 页码 -出版社
WILEY
DOI: 10.1111/and.12842
关键词
in vitro fertilisation; nanomicelle curcumin; rat; sperm quality; spermatogenesis
类别
资金
- department of Biology, Faculty of Basic science and Urmia University
This study was conducted to investigate the possible effects of nanomicelle curcumin (NMC) on spermatogenesis, sperm parameters and in vitro fertilisation potential. For this purpose, 24 mature male Wistar rats were divided into control and test groups. The animals in test groups received 7.5, 15 and 30mgkgb.w(-1) of NMC (NO=6 rats in each group). Following 48days, the DNA integrity of testicular tissues, tubular differentiation (TDI) and spermiogenesis (SPI) indices, sperm parameters and DNA integrity were analysed. Finally, the in vitro fertilisation potential was investigated via evaluating pre-implantation embryo generation. The NMC diminished the TDI and SPI ratios. The animals in NMC-received groups exhibited a remarkable (p<.05) reduction in percentage of alive and motile spermatozoa. Moreover, the NMC enhanced the percentage of spermatozoa with decondensed chromatin and elevated the sperm DNA damage ratio. The testicles of NMC-received groups exhibited severe DNA fragmentation. The percentages of zygote, 2-cell, blastocysts and hatched embryos generation were decreased in NMC-received groups compared to control animals. In conclusion, the NMC adversely affects the spermatogenesis and spermiogenesis processes, which in turn results in reducing the sperm quality. Ultimately, decreased sperm quality results in lower pre-implantation embryo development.
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