期刊
EUROPEAN JOURNAL OF IMMUNOLOGY
卷 48, 期 2, 页码 283-292出版社
WILEY
DOI: 10.1002/eji.201646861
关键词
CD38; Class switch recombination; CpG-ODN; Plasma cell; Retinoic acid
类别
资金
- Technology Foundation STW (part of NWO), the Netherlands [13017]
Class-switching of Bcells to IgA can be induced via both T-cell-dependent and T-cell-independent mechanisms. IgA is most predominantly produced mucosally and is important for combating infections and allergies. In contrast to mice, humans have two forms of IgA; IgA1 and IgA2 with diverse tissue distribution. In early life, IgA levels might be sub-optimal especially during the fall season when bacterial and viral infections are more common. Therefore, we investigated using human Bcells whether T-cell-independent factors -promoting cell survival, class switching and immunoglobulin secretion- BAFF, APRIL, IL-10 and retinoic acid can boost IgA production in the context of viral or bacterial infection. To this end total and naive peripheral blood Bcells were stimulated with these factors for 6 days in the presence or absence of TLR7/8 agonist R848 (mimicking viral infection) or TLR9 agonist CpG-ODN (mimicking bacterial infection). We show that BAFF significantly augments IgA2 production in TLR7/8 stimulated mature, but not naive Bcells. In addition, BAFF augments IL-10 production and viability in TLR7/8 and TLR9 stimulated mature Bcells. These data warrant further investigation of its role in immune regulation both in the periphery and mucosal tissues in early life or during disease.
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