期刊
RNA
卷 24, 期 2, 页码 143-148出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.061945.117
关键词
SHAPE; RING; 1M7; NMIA; 1M6; 5NIA; NAI; dimethyl sulfate; single molecule; correlated chemical probing; mutational profiling; RNA structure modeling
资金
- Moderna Therapeutics
- National Institutes of Health
- Office of Extramural Research [AI068462]
Mutational profiling (MaP) enables detection of sites of chemical modification in RNA as sequence changes during reverse transcription (RT), subsequently read out by massively parallel sequencing. We introduce ShapeMapper 2, which integrates careful handling of all classes of adduct-induced sequence changes, sequence variant correction, basecall quality filters, and quality-control warnings to now identify RNA adduct sites as accurately as achieved by careful manual analysis of electrophoresis data, the prior highest-accuracy standard. MaP and ShapeMapper 2 provide a robust, experimentally concise, and accurate approach for reading out nucleic acid chemical probing experiments.
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