4.4 Article

Dual nature of pseudouridylation in U2 snRNA: Pus1 p-dependent and Pus1 p-independent activities in yeasts and higher eukaryotes

期刊

RNA
卷 23, 期 7, 页码 1060-1067

出版社

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.061226.117

关键词

modification guide RNA; pseudouridine; Pus1p; U2 snRNA

资金

  1. National Institute of General Medical Sciences of the National Institutes of Health [R01 GM33397]

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The pseudouridine at position 43 in vertebrate U2 snRNA is one of the most conserved post -transcriptional modifications of spliceosomal snRNAs; the equivalent position is pseudouridylated in U2 snRNAs in different phyla including fungi, insects, and worms. Pseudouridine synthase Pus1p acts alone on U2 snRNA to form this pseudouridine in yeast Saccharomyces cerevisiae and mouse. Furthermore, in S. cerevisiae, Pus1p is the only pseudouridine synthase for this position. Using an in vivo yeast cell system, we tested enzymatic activity of Pus1p from the fission yeast Schizosaccharomyces pombe, the worm Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the frog Xenopus tropicalis. We demonstrated that Pus1 Delta from C. elegans has no enzymatic activity on U2 snRNA when expressed in yeast cells, whereas in similar experiments, position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p from S. cerevisiae, S. pombe, Drosophila, Xenopus, and mouse. However, when we analyzed U2 snRNAs from Pusl knockout mice and the pus14 S. pombe strain, we could not detect any changes in their modification patterns when compared to wild -type U2 snRNAs. In S. pombe, we found a novel box H/ACA RNA encoded downstream from the RPC10 gene and experimentally verified its guide RNA activity for positioning psi 43 and psi 44 in U2 snRNA. In vertebrates, we showed that SCARNA8 (also known as U92 scaRNA) is a guide for U2 -psi 43 in addition to its previously established targets U2-psi 34/psi 44.

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