期刊
RNA BIOLOGY
卷 14, 期 11, 页码 1627-1637出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2017.1342933
关键词
6S RNA; high salt stress; Northern blot analysis; (photo)oxidative stress; pRNA synthesis; RNA-Seq; ssrS deletion
资金
- Deutsche Forschungsgemeinschaft [IRTG 1384]
- Russian Scientific Foundation [14-24-00061]
- Russian Science Foundation [17-24-00003] Funding Source: Russian Science Foundation
The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic alpha-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12-16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA >= 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the Delta ssrS strain when grown in the presence of 250 mM NaCl.
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