期刊
CLINICAL INFECTIOUS DISEASES
卷 66, 期 5, 页码 778-788出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/cid/cix881
关键词
metagenomics; diagnosis; next-generation sequencing; bioinformatics
资金
- Accelerate Diagnostics
- Curetis
- BD Diagnostics
- GenePOC
- Roche Molecular
- McGraw-Hill
- American Society for Microbiology
- Elsevier
- bioMerieux
- Cellex
- Check-Points Diagnostics
- BV
- Hardy Diagnostics
Agnostic metagenomic next-generation sequencing (mNGS) has emerged as a promising single, universal pathogen detection method for infectious disease diagnostics. This methodology allows for identification and genomic characterization of bacteria, fungi, parasites, and viruses without the need for a priori knowledge of a specific pathogen directly from clinical specimens. Although there are increasing reports of mNGS successes, several hurdles need to be addressed, such as differentiation of colonization from infection, extraneous sources of nucleic acid, method standardization, and data storage, protection, analysis, and interpretation. As more commercial and clinical microbiology laboratories develop mNGS assays, it is important for treating practitioners to understand both the power and limitations of this method as a diagnostic tool for infectious diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据