期刊
VETERINARY RESEARCH
卷 49, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13567-018-0513-2
关键词
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资金
- China Postdoctoral Science Foundation [2017M611933]
- Yangzhou university veterinary department of scientific research foundation for postdoctoral [137070355]
- Yangzhou university innovative entrepreneurial training program for students [x20170752]
- National Natural Science Foundation of China [31472171, 31402228]
- Key University Science Research Project of Jiangsu Province [14KJA230002]
- Research Foundation for Talented Scholars in Yangzhou University
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Cell-penetrating peptide (CPP) is a promising cargo for delivering bioactive molecules. In this study, the N terminus of VP1 from chicken anemia virus, designated as CVP1, was found to carry enriched arginine residues with alpha-helix. By confocal imaging, flow cytometry and MTT assay, we identified CVP1 as a novel, safe and efficient CPP. CVP1-FITC peptide could entry different types of cells tested with dose dependence, but without cytotoxic effects. Compared with TAT-FITC peptide, the CVP1-FITC peptide showed much higher cell-penetrating activity. Moreover, CVP1 could successfully deliver ss-glycosidase, poly (I:C) and plasmid into HCT116 cells. Inhibitors and temperature sensitivity analysis further indicated that the cell-penetrating activity of CVP1 was based on ATP-dependent and caveolae-mediated endocytosis. All these data demonstrate that CVP1 has efficient cell-penetrating activity and great potential for developing a novel delivery vector.
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