期刊
ENVIRONMENTAL POLLUTION
卷 203, 期 -, 页码 97-106出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2015.03.042
关键词
Bioremediation; 1,2-Dichloroethane (DCA); Groundwater contamination; Real-time polymerase chain reaction (PCR)
资金
- Taiwan National Science Council [99-2221-E-110-027-MY3]
- Nan-Tz Industrial Park, Kaohsiung, Taiwan
In this study, the effectiveness of bioremediating 1,2-dichloroethane (DCA)-contaminated groundwater under different oxidation reduction processes was evaluated. Microcosms were constructed using indigenous bacteria and activated sludge as the inocula and cane molasses and a slow polycolloid-releasing substrate (SPRS) as the primary substrates. Complete DCA removal was obtained within 30 days under aerobic and reductive dechlorinating conditions. In anaerobic microcosms with sludge and substrate addition, chloroethane, vinyl chloride, and ethene were produced. The microbial communities and DCA-degrading bacteria in microcosms were characterized by 16S rRNA-based denatured-gradient-gel electrophoresis profiling and nucleotide sequence analyses. Real-time polymerase chain reaction was applied to evaluate the variations in Dehalococcoides spp. and Desulfitobacterium spp. Increase in Desulfitobacterium spp. indicates that the growth of Desulfirobacterium might be induced by DCA. Results indicate that DCA could be used as the primary substrate under aerobic conditions. The increased ethene concentrations imply that dihaloelimination was the dominate mechanism for DCA biodegradation. (C) 2015 Elsevier Ltd. All rights reserved.
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