期刊
RADIOTHERAPY AND ONCOLOGY
卷 124, 期 3, 页码 439-447出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.radonc.2017.04.001
关键词
Eukaryotic elongation factor 2 kinase; Esophageal squamous cell carcinoma; Radiosensitivity; Autophagy; Apoptosis; Tumor growth
资金
- Natural Science Foundation of China [81672983, 81502653, 81472809, 81272504]
- Innovation Team [LJ201123 (EH11)]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) [JX10231801]
- Key Academic Discipline of Jiangsu Province Medical Aspects of Specific Environments, Research and Innovation Project for College Graduates of Jiangsu Province [KYLX_0955]
Objectives: To investigate the biological function of eEF2K in esophageal squamous cell carcinoma (ESCC). Materials and methods: Tissue microarrays containing 100 pairs of ESCC tumor and adjacent normal tissues were completed. Overexpression and knockdown of eEF2K were constructed in ECA-109 and TE-13 ESCC cells. DNA damage, cell viability, migration and invasion, radioresistance, apoptosis and autophagy were determined by immunofluorescence, CCK-8, transwell assay, colony formation assay, flow cytometry and western blot, respectively. Tumor growth and radioresistance were also evaluated using xenograft models created in nude mice. Results: eEF2K expression was higher in ESCC tissues compared with matched non-tumor tissues (P < 0.05). Proliferation was increased in eEF2K overexpressing cells compared with controls (P < 0.05), while silencing eEF2K reduced cell proliferation (P < 0.05). Furthermore, lower levels of eEF2K expression correlated with slower migration and invasion rates (P < 0.05), while higher levels of eEF2K expression with faster migration and invasion rates (P < 0.05). eEF2K overexpression resulted in radioresistance and radiation-induced autophagy, and reduced radiation-induced apoptosis compared with controls, but silencing eEF2K promoted radiosensitivity and apoptosis, and reduced autophagy. In addition, eEF2K overexpression promoted the tumor growth in vivo (P < 0.01). Combined treatment of NH125 (a pharmacological inhibitor of eEF2K) and radiation was more effective at delaying xenograft tumor growth than NH125 and radiation alone (P < 0.05). Conclusion: eEF2K induced progression and radioresistance in ESCC, which may be a novel therapeutic target for ESCC to increase radiosensitivity. (C) 2017 Elsevier B.V. All rights reserved.
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