期刊
DNA RESEARCH
卷 25, 期 2, 页码 183-194出版社
OXFORD UNIV PRESS
DOI: 10.1093/dnares/dsx048
关键词
PRDM9; recombination; cattle; allele; single sperm genomics
资金
- AFRI grant from the USDA NIFA [2011-67015-30183, 2013-67015-20951, 2016-67015-24886]
- US-Israel Binational Agricultural Research and Development Fund [US-4997-17]
- Agricultural Research Service of the United States Department of Agriculture [8042-31000-104-00, 1265-31000-096-00]
- NIFA [579688, 688112, 2011-67015-30183, 2013-67015-20951] Funding Source: Federal RePORTER
PRDM9 contributes to hybrid sterility and species evolution. However, its role is to be confirmed in cattle, a major domesticated livestock species. We previously found an association near PRDM9 with cattle recombination features, but the causative variants are still unknown. Using millions of genotyped cattle with pedigree information, we characterized five PRDM9 alleles and generated allele-specific recombination maps. By examining allele-specific recombination patterns, we observed the impact of PRDM9 on global distribution of recombination, especially in the two ends of chromosomes. We also showed strong associations between recombination hot-spot regions and functional mutations within PRDM9 zinc finger domain. More importantly, we found one allele of PRDM9 to be very different from others in both protein composition and recombination landscape, indicating the causative role of this allele on the association between PRDM9 and cattle recombination. When comparing recombination maps from sperm and pedigree data, we observed similar genome-wide recombination patterns, validating the quality of pedigree-based results. Collectively, these evidence supported PRDM9 alleles as causal variants for the reported association with cattle recombination. Our study comprehensively surveyed the bovine PRDM9 alleles, generated allele-specific recombination maps, and expanded our understanding of the role of PRDM9 on genome distribution of recombination.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据