期刊
ENDOCRINOLOGY
卷 159, 期 3, 页码 1393-1400出版社
OXFORD UNIV PRESS INC
DOI: 10.1210/en.2017-03076
关键词
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资金
- National Institute of Diabetes and Digestive and Kidney Diseases [DK-020595, DK-072473]
- National Institute on Aging [AG-042151]
- Kovler Family Foundation
- National Institute of Biomedical Imaging and Bioengineering [K99EB022636]
Pancreatic islets are endocrine micro-organs scattered throughout the exocrine pancreas. Islets are surrounded by a network of vasculature, ducts, neurons, and extracellular matrix. Three-dimensional imaging is critical for such structural analyses. We have adapted transparent tissue tomography to develop a method to image thick pancreatic tissue slices (1 mm) with multi-fluorescent channels. This method takes only 2 to 3 days from specimen preparation and immunohistochemical staining to clearing tissues and imaging. Reconstruction of the intact pancreas visualizes islets with beta, alpha, and delta cells together with their surrounding networks. Capturing several hundred islets at once ensures sufficient power for statistical analyses. Further surface rendering provides clear views of the anatomical relationship between islets and their microenvironment as well as the basis for volumetric quantification. As a proof-of-principle demonstration, we show an islet size-dependent increase of intraislet capillary density and an inverse decrease in sphericity.
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