期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 185, 期 1, 页码 163-178出版社
SPRINGER
DOI: 10.1007/s12010-017-2644-8
关键词
Candida intermedia; Single-cell protein; Xylitol; Lignocellulosic hydrolysate; Simultaneous saccharification and fermentation
资金
- Special Fund for Agro-scientific Research in the Public Interest [201503137]
- Fundamental Research Funds for Central Non-profit Scientific Institution [1610012017010, Y2017JC39]
Yeasts are good candidates to utilize the hydrolysates of lignocellulose, the most abundant bioresource, for bioproducts. This study aimed to evaluate the efficiencies of single-cell protein (SCP) and xylitol production by a novel yeast strain, Candida intermedia FL023, from lignocellulosic hydrolysates and xylose. This strain efficiently assimilated hexose, pentose, and cellubiose for cell mass production with the crude protein content of 484.2 g kg(-1) dry cell mass. SCP was produced by strain FL023 using corncob hydrolysate and urea as the carbon and nitrogen sources with the dry cell mass productivity 0.86 g L-1 h(-1) and the yield of 0.40 g g(-1) sugar. SCP was also produced using NaOH-pretreated Miscanthus sinensis straw and corn steep liquor as the carbon and nitrogen sources through simultaneous saccharification and fermentation with the dry cell productivity of 0.23 g L-1 h(-1) and yield of 0.17 g g(-1) straw. C. intermedia FL023 was tolerant to 0.5 g L-1 furfural, acetic acid, and syringaldehyde in xylitol fermentation and produced 45.7 g L-1 xylitol from xylose with the productivity of 0.38 g L-1 h(-1) and the yield of 0.57 g g(-1) xylose. This study provides feasible methods for feed and food additive production from the abundant lignocellulosic bioresources.
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