3.8 Article Book Chapter

Nonradioactive Northern Blot of circRNAs

期刊

CIRCULAR RNAS: METHODS AND PROTOCOLS
卷 1724, 期 -, 页码 135-141

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HUMANA PRESS INC
DOI: 10.1007/978-1-4939-7562-4_11

关键词

Nonradioactive; Northern blot; circRNA; Digoxigenin

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Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.

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