Stretch-activated two-pore-domain (K2P) potassium channels in the heart: Focus on atrial fibrillation and heart failure

Two-pore-domain potassium (K-2P) channels modulate cellular excitability. The significance of stretch activated cardiac K-2P channels (K(2P)2.1, TREK-I, KCNK2; K(2P)4.1, TRAAK, KCNK4; K(2P)10.1, TREK-2, KCNIC10) in heart disease has not been elucidated in detail. The aim of this work was to assess expression and remodeling of mechanosensitive K-2P channels in atrial fibrillation (AF) and heart failure (HF) patients in comparison to murine models. Cardiac K-2P channel levels were quantified in atrial (A) and ventricular (V) tissue obtained from patients undergoing open heart surgery. In addition, control mice and mouse models of AF (cAMP-response element modulator (CREM)-Ib Delta C-X transgenic animals) or HF (cardiac dysfunction induced by transverse aortic constriction, TAC) were employed. Human and murine KCNK2 displayed highest mRNA abundance among mechanosensitive members of the K-2P channel family (V > A). Disease-associated K(2P)2.1 remodeling was studied in detail. In patients with impaired left ventricular function, atrial ICCNIC2 (K(2P)2.1) mRNA and protein expression was significantly reduced. In AF subjects, downregulation of atrial and ventricular KCNK2 (K(2P)2.1) mRNA and protein levels was observed. AF-associated suppression of atrial Kcnk2 (K(2P)2.1) mRNA and protein was recapitulated in CREM-transgenic mice. Ventricular Kcnk2 expression was not significantly altered in mouse models of disease. In conclusion, mechanosensitive K(2P)2.1 and K(2P)10.1 K+ channels are expressed throughout the heart. HF- and AF-associated downregulation of KCNIC2 (K(2P)2.1) mRNA and protein levels suggest a mechanistic contribution to cardiac arrhythmogenesis. (C) 2017 Elsevier Ltd. All rights reserved.