期刊
PROCESS BIOCHEMISTRY
卷 57, 期 -, 页码 87-94出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.03.026
关键词
Enzyme-inorganic nanoflowers; Microbeads; Alginate; Enzyme immobilization; Diacetyl
资金
- National Natural Science Foundation of China [21433001, 21603251]
- Applied Basic Research Foundation of Qingdao City [14-2-4-21-jch]
In this work, novel nanoflower/alginate microbeads were synthesized to immobilize alpha-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca-3(PO4)(2) to form enzyme inorganic hybrid nanoflowers (Ca-3(PO4)(2)-ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca-3(PO4)(2)-ALDC)@Mg). Compared with free ALDC and Ca-3(PO4)(2)-ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300 L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry.
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