期刊
PROCESS BIOCHEMISTRY
卷 58, 期 -, 页码 204-210出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2017.04.011
关键词
High hydrostatic pressure; Enzymatic hydrolysis; Pretreatment; Ferulic acid; Corn cob
资金
- Spanish Ministry of Science and Innovation [CTQ2015-71436-C2-1-R]
- ERDF funds of the European Union
- Spanish Ministry of Education, Culture and Sports
Bioprocesses based on the use of enzymes can be employed for the obtainment of ferulic acid by its solubilization from corn cob, which is a natural source of this phenolic compound. In order to overcome the limitations caused by the recalcitrance of biomass, the application of pretreatments is advisable. The utilization of high hydrostatic pressure on the enzymatic release of ferulic acid from corn cob with three feruloyl esterase extracts was studied applying pressurization at 600 MPa as a pretreatment in combination with a mild thermal treatment at 130 degrees C/2 h. Additionally, pressurization at 200 MPa during the enzymatic hydrolysis step was assessed to increase the ferulic acid yield. The results showed that the application of a high pressure pretreatment at 600 MPa/40 degrees C/15 min to thermally treated corn cob increased by 20% the ferulic acid release in comparison with the thermal treatment for Ultraflo (R) L, but not for the Aspergillus extracts. Pressurization at 200 MPa during the hydrolysis stage affected differently the enzymes stability and ferulic acid release depending on the extract and the pretreatments applied, showing that the effects of pressurization are complex and must be assessed in each case.
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