期刊
PROCESS BIOCHEMISTRY
卷 53, 期 -, 页码 109-115出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2016.12.012
关键词
Feruloyl esterases; Bacillus
资金
- National Key Scientific Instrument and Equipment Development Project of China [2013YQ17052504]
- Program for Changjiang Scholars and Innovative Research Team in the University of Ministry of Education of China [IRT_15R55]
Feruloyl esterases (Faes) are a subclass of the carboxylic esterases that hydrolyze the ester bonds between ferulic acid and polysaccharides in plant cell walls. Until now, the biochemical characteristics of FAEs from Bacillus spp. have not been reported. In this study, a strain with high activity of FAEs, Bacillus amyloliquefaciens H47 was screened from 122 Bacillus - type strains. Finally, three FAE5 (BaFae04, BaFae06, and BaFae09) were identified. Comparing with other bacterial FAEs, these novel FAE5 exhibited low sequence identities (less than 30%). The profiles of 52 esterase substrates showed that the three FAEs had a broad substrate spectrum and could effectively hydrolyze several common FAE substrates, such as methyl ferulate, ethyl caffeate, methyl p-coumarate, methyl sinapate, and chlorogenic acid. Furthermore, the three FAEs also can release ferulic acid from destarched wheat bran. They Showed maximal activity with an optimal pH of 8.0 at 30 degrees C, 35 degrees C, and 40 degrees C, respectively. BaFae04 showed high stability in the temperature range of 25-60 degrees C for 1 h and retained 59% of its activity at 60 degrees C. The present study displays some useful characteristics of FAEs for potential industrial application and contributes to our understanding of FAEs. (C) 2016 Elsevier Ltd. All rights reserved.
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