期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 28, 页码 E5750-E5759出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1700054114
关键词
cell polarization; cell migration; mechanochemical feedback; Rho-family small GTPases; extracellular matrix
资金
- Samsung scholarship
- NIH [U54 CA209992, U01 CA155758]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-41870]
- NSF [DMS-1562078]
- Division Of Mathematical Sciences
- Direct For Mathematical & Physical Scien [1562078] Funding Source: National Science Foundation
Cell polarization and directional cell migration can display random, persistent, and oscillatory dynamic patterns. However, it is not clear whether these polarity patterns can be explained by the same underlying regulatory mechanism. Here, we show that random, persistent, and oscillatory migration accompanied by polarization can simultaneously occur in populations of melanoma cells derived from tumors with different degrees of aggressiveness. We demonstrate that all of these patterns and the probabilities of their occurrence are quantitatively accounted for by a simple mechanism involving a spatially distributed, mechanochemical feedback coupling the dynamically changing extracellular matrix (ECM)-cell contacts to the activation of signaling downstream of the Rho-family small GTPases. This mechanism is supported by a predictive mathematical model and extensive experimental validation, and can explain previously reported results for diverse cell types. In melanoma, this mechanism also accounts for the effects of genetic and environmental perturbations, including mutations linked to invasive cell spread. The resulting mechanistic understanding of cell polarity quantitatively captures the relationship between population variability and phenotypic plasticity, with the potential to account for a wide variety of cell migration states in diverse pathological and physiological conditions.
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