期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 34, 页码 9200-9205出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1704754114
关键词
aquaporin; pathogen; guard cell signaling; hydrogen peroxide; stomatal movement
资金
- French Ministry of Higher Education and Research
- Erasmus Mundus EUROEAST grant from the European Commission
Stomatal movements are crucial for the control of plant water status and protection against pathogens. Assays on epidermal peels revealed that, similar to abscisic acid (ABA), pathogen-associated molecular pattern (PAMP) flg22 requires the AtPIP2; 1 aquaporin to induce stomatal closure. Flg22 also induced an increase in osmotic water permeability (P-f) of guard cell protoplasts through activation of AtPIP2; 1. The use of HyPer, a genetic probe for intracellular hydrogen peroxide (H2O2), revealed that both ABA and flg22 triggered an accumulation of H2O2 in wild-type but not pip2; 1 guard cells. Pre-treatment of guard cells with flg22 or ABA facilitated the influx of exogenous H2O2. Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) and open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6) were both necessary to flg22-induced P-f and both phosphorylated AtPIP2; 1 on Ser121 in vitro. Accumulation of H2O2 and stomatal closure as induced by flg22 was restored in pip2; 1 guard cells by a phosphomimetic form (Ser121Asp) but not by a phosphodeficient form (Ser121Ala) of AtPIP2; 1. We propose amechanismwhereby phosphorylation of AtPIP2; 1 Ser121 by BAK1 and/or OST1 is triggered in response to flg22 to activate its water and H2O2 transport activities. This work establishes a signaling role of plasmamembrane aquaporins in guard cells and potentially in other cellular context involving H2O2 signaling.
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