期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 23, 页码 5988-5993出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1701425114
关键词
International Space Station; preservation; freeze-dry; spermatozoa; fertilization
资金
- Japan Society for the Promotion of Science (JSPS)
- JSPS [26506006]
- Naito Foundation
- Asada Science Foundation
- Takeda Science Foundation
- Japan Aerospace Exploration Agency
- Grants-in-Aid for Scientific Research [26506006, 16H02478, 26506032, 16J07536] Funding Source: KAKEN
If humans ever start to live permanently in space, assisted reproductive technology using preserved spermatozoa will be important for producing offspring; however, radiation on the International Space Station (ISS) is more than 100 times stronger than that on Earth, and irradiation causes DNA damage in cells and gametes. Here we examined the effect of space radiation on freeze-dried mouse spermatozoa held on the ISS for 9 mo at -95 degrees C, with launch and recovery at room temperature. DNA damage to the spermatozoa and male pronuclei was slightly increased, but the fertilization and birth rates were similar to those of controls. Next-generation sequencing showed only minor genomic differences between offspring derived from space-preserved spermatozoa and controls, and all offspring grew to adulthood and had normal fertility. Thus, we demonstrate that although space radiation can damage sperm DNA, it does not affect the production of viable offspring after at least 9 mo of storage on the ISS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据