期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 44, 页码 E9271-E9279出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1703921114
关键词
autophagy; melanoma; natural killer; CCL5; immunotherapy
资金
- Fondation Cancer, Luxembourg [FC/2012/02, F/2016/01]
- Institut National du Cancer
- Ligue Contre le Cancer (Equipes Labellisees)
- Fonds de la Recherche Scientifique FNRS Televie [7.6503.16, 7.4664.15, 7.4571.15]
- Luxembourg Institute of Health [LHCE 2013 11 05]
- Fond National de la Recherche, Luxembourg [FNR-AFR 7842786]
- Calouste Gulbenkian Foundation [P.1333237]
While blocking tumor growth by targeting autophagy is well established, its role on the infiltration of natural killer (NK) cells into tumors remains unknown. Here, we investigate the impact of targeting autophagy gene Bedin1 (BECN1) on the infiltration of NK cells into melanomas. We show that, in addition to inhibiting tumor growth, targeting BECN1 increased the infiltration of functional NK cells into melanoma tumors. We provide evidence that driving NK cells to the tumor bed relied on the ability of autophagy-defective tumors to transcriptionally overexpress the chemokine gene CCL5. Such infiltration and tumor regression were abrogated by silencing CCL5 in BECN1-defective tumors. Mechanistically, we show that the up-regulated expression of CCL5 occurred through the activation of its transcription factor c-Jun by a mechanism involving the impairment of phosphatase PP2A catalytic activity and the subsequent activation of JNK. Similar to BECN1, targeting other autophagy genes, such as ATG5, p62/SQSTM1, or inhibiting autophagy pharmacologically by chloroquine, also induced the expression of CCL5 in melanoma cells. Clinically, a positive correlation between CCL5 and NK cell marker NKp46 expression was found in melanoma patients, and a high expression level of CCL5 was correlated with a significant improvement of melanoma patients' survival. We believe that this study highlights the impact of targeting autophagy on the tumor infiltration by NK cells and its benefit as a novel therapeutic approach to improve NK-based immunotherapy.
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