期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 36, 页码 E7622-E7631出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1710848114
关键词
CD146; claudin-5; PDGFR beta; spatiotemporal expression; blood-brain barrier
资金
- National Basic Research Program of China 973 Programs [2015CB553705, 2014CBA02003]
- National Natural Science Foundation of China [31370824, 81371025, 91529306, 81371330, 91640112, 31670185, 81527805, 81671851, 81502547]
- Chinese Academy of Sciences [XDA12020207, XDA12030202]
- Guangdong Natural Science Fund for Distinguished Young Scholars, China
- Novo Nordisk-Chinese Academy of Sciences [NNCAS-2015-11]
The blood-brain barrier (BBB) establishes a protective interface between the central neuronal system and peripheral blood circulation and is crucial for homeostasis of the CNS. BBB formation starts when the endothelial cells (ECs) invade the CNS and pericytes are recruited to the nascent vessels during embryogenesis. Despite the essential function of pericyte-EC interaction during BBB development, the molecular mechanisms coordinating the pericyte-EC behavior and communication remain incompletely understood. Here, we report a single cell receptor, CD146, that presents dynamic expression patterns in the cerebrovasculature at the stages of BBB induction and maturation, coordinates the interplay of ECs and pericytes, and orchestrates BBB development spatiotemporally. In mouse brain, CD146 is first expressed in the cerebrovascular ECs of immature capillaries without pericyte coverage; with increased coverage of pericytes, CD146 could only be detected in pericytes, but not in cerebrovascular ECs. Specific deletion of Cd146 in mice ECs resulted in reduced brain endothelial claudin-5 expression and BBB breakdown. By analyzing mice with specific deletion of Cd146 in pericytes, which have defects in pericyte coverage and BBB integrity, we demonstrate that CD146 functions as a coreceptor of PDGF receptor-beta to mediate pericyte recruitment to cerebrovascular ECs. Moreover, we found that the attached pericytes in turn down-regulate endothelial CD146 by secreting TGF-beta 1 to promote further BBB maturation. These results reveal that the dynamic expression of CD146 controls the behavior of ECs and pericytes, thereby coordinating the formation of a mature and stable BBB.
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