期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 8, 页码 1789-1794出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1613541114
关键词
sensors; imaging; dopamine; carbon nanotube; chemical signaling
资金
- Deutsche Forschungsgemeinschaft
- Liebig Fellowship (Fonds der chemischen Industrie)
- National Science Foundation (NSF) [2388357]
- NSF [PHY1430124]
- NIH Grant [9P41GM104601]
- McGovern Institute Neurotechnology Program
Intercellular communication via chemical signaling proceeds with both spatial and temporal components, but analytical tools, such as microfabricated electrodes, have been limited to just a few probes per cell. In this work, we use a nonphotobleaching fluorescent nanosensor array based on single-walled carbon nanotubes (SWCNTs) rendered selective to dopamine to study its release from PC12 neuroprogenitor cells at a resolution exceeding 20,000 sensors per cell. This allows the spatial and temporal dynamics of dopamine release, following K+ stimulation, to be measured at exceedingly high resolution. We observe localized, unlabeled release sites of dopamine spanning 100 ms to seconds that correlate with protrusions but not predominately the positive curvature associated with the tips of cellular protrusions as intuitively expected. The results illustrate how directionality of chemical signaling is shaped by membrane morphology, and highlight the advantages of nanosensor arrays that can provide high spatial and temporal resolution of chemical signaling.
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