期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 12, 页码 3175-3180出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1618088114
关键词
phosphoproteins; proteomics; extracellular vesicles; mass spectrometry; biomarker
资金
- NIH [5R41GM109626, 1R01GM111788, 1R41CA210772]
- National Science Foundation [1506752]
- Purdue University Center for Cancer Research (NIH Grant) [P30 CA023168]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1506752] Funding Source: National Science Foundation
The state of protein phosphorylation can be a key determinant of cellular physiology such as early-stage cancer, but the development of phosphoproteins in biofluids for disease diagnosis remains elusive. Here we demonstrate a strategy to isolate and identify phosphoproteins in extracellular vesicles (EVs) from human plasma as potential markers to differentiate disease from healthy states. We identified close to 10,000 unique phosphopeptides in EVs isolated from small volumes of plasma samples. Using label-free quantitative phosphoproteomics, we identified 144 phosphoproteins in plasma EVs that are significantly higher in patients diagnosed with breast cancer compared with healthy controls. Several biomarkers were validated in individual patients using paralleled reaction monitoring for targeted quantitation. This study demonstrates that the development of phosphoproteins in plasma EV as disease biomarkers is highly feasible and may transform cancer screening and monitoring.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据