期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 12, 页码 3133-3138出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1614145114
关键词
centromere; kinetochore; CENP-A; centrochromatin; boustrophedon
资金
- Wellcome Trust [107022, 203149, WT104915MA]
- Japan Society for Promotion of Science KAKENHI Grants [JP25221106, JP15H05972]
- Darwin Trust of Edinburgh
- Grants-in-Aid for Scientific Research [17H06167, 15H05972] Funding Source: KAKEN
During cell division, interactions between microtubules and chromosomes are mediated by the kinetochore, a proteinaceous structure located at the primary constriction of chromosomes. In addition to the centromere histone centromere protein A (CENP-A), 15 other members of the constitutive centromere associated network (CCAN) participate in the formation of a chromatin-associated scaffold that supports kinetochore structure. We performed a targeted screen analyzing unfolded centrochromatin from CENP-depleted chromosomes. Our results revealed that CENP-C and CENP-S are critical for the stable folding of mitotic kinetochore chromatin. Multipeak fitting algorithms revealed the presence of an organized pattern of centrochromatin packing consistent with arrangement of CENPA- containing nucleosomes into up to five chromatin subunits each containing roughly 20-30 nucleosomes. These subunits could be either layers of a boustrophedon or small loops of centromeric chromatin.
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