期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 38, 页码 E7919-E7928出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1708142114
关键词
biomaterials; scaffold; muscle stem cell; cell delivery; muscle regeneration
资金
- National Cancer Institute Cancer Center Support Grant [P30 CA060553]
- Soft and Hybrid Nanotechnology Experimental Resource [National Science Foundation (NSF) ] [NNCI-1542205]
- Materials Research Science and Engineering Centers program at the Materials Research Center [NSF DMR-1121262]
- International Institute for Nanotechnology (IIN)
- Keck Foundation
- State of Illinois, through the IIN
- NIH through National Heart, Lung, and Blood Institute (NHLBI) PPG Grant [1P01HL108795]
- NIH/NHLBI Bioengineering Research Partnership Grant [5R01HL116577]
- Louis A. Simpson and Kimberly K. Querrey Center for Regenerative Nanomedicine
- Baxter Foundation
- California Institute for Regenerative Medicine [RB5-07469]
- NIH [AG020961, K99AG042491, R00AG042491]
Muscle stem cells are a potent cell population dedicated to efficacious skeletal muscle regeneration, but their therapeutic utility is currently limited by mode of delivery. We developed a cell delivery strategy based on a supramolecular liquid crystal formed by peptide amphiphiles (PAs) that encapsulates cells and growth factors within a muscle-like unidirectionally ordered environment of nanofibers. The stiffness of the PA scaffolds, dependent on amino acid sequence, was found to determine the macroscopic degree of cell alignment templated by the nanofibers in vitro. Furthermore, these PA scaffolds support myogenic progenitor cell survival and proliferation and they can be optimized to induce cell differentiation and maturation. We engineered an in vivo delivery system to assemble scaffolds by injection of a PA solution that enabled coalignment of scaffold nanofibers with endogenous myofibers. These scaffolds locally retained growth factors, displayed degradation rates matching the time course of muscle tissue regeneration, and markedly enhanced the engraftment of muscle stem cells in injured and noninjured muscles in mice.
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