期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 114, 期 12, 页码 E2476-E2485出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1616815114
关键词
circadian rhythm; membrane potential; intracellular calcium; time-lapse imaging; neuronal network
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science Research [25860175, 25113701, 15KT0072, 24390055, 15H04679, 16K16645, 16H05120]
- Precursory Research for Embryonic Science and Technology/Japan Science and Technology Agency
- Cooperative Research Project for Advanced Photonic Bioimaging
- Project for Developing Innovation Systems of MEXT
- Akiyama Life Science Foundation
- Japan Foundation for Neuroscience and Mental Health
- Narishige Neuroscience Research Foundation
- Grants-in-Aid for Scientific Research [16H05120, 24390055, 16K16645, 16H01608, 17K08561, 15K15039, 25113701, 15H04679, 15K12763, 15KT0072, 26860156, 25860175] Funding Source: KAKEN
The suprachiasmatic nucleus (SCN), the master circadian clock, contains a network composed of multiple types of neurons which are thought to form a hierarchical and multioscillator system. The molecular clock machinery in SCN neurons drives membrane excitability and sends time cue signals to various brain regions and peripheral organs. However, how and at what time of the day these neurons transmit output signals remain largely unknown. Here, we successfully visualized circadian voltage rhythms optically for many days using a genetically encoded voltage sensor, ArcLightD. Unexpectedly, the voltage rhythms are synchronized across the entire SCN network of cultured slices, whereas simultaneously recorded Ca2+ rhythms are topologically specific to the dorsal and ventral regions. We further found that the temporal order of these two rhythms is cell-type specific: The Ca2+ rhythms phase-lead the voltage rhythms in AVP neurons but Ca2+ and voltage rhythms are nearly in phase in VIP neurons. We confirmed that circadian firing rhythms are also synchronous and are coupled with the voltage rhythms. These results indicate that SCN networks with asynchronous Ca2+ rhythms produce coherent voltage rhythms.
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