4.8 Article

A stromal region of cytochrome b6f subunit IV is involved in the activation of the Stt7 kinase in Chlamydomonas

出版社

NATL ACAD SCIENCES
DOI: 10.1073/pnas.1713343114

关键词

cytochrome b(6)f complex; structure-function; random mutagenesis; Stt7 kinase; state transitions

资金

  1. IRTELIS PhD Grant from CEA
  2. Initiative d'Excellence program (Grant DYNAMO) [ANR-11-LABEX-0011-01]
  3. Agence Nationale de la Recherche [ChloroPaths: ANR-14-CE05-0041-01]
  4. CNRS
  5. INSIS (Institut des Sciences de l'Ingenierie et des Systemes) Energie CNRS [PhotoModes 72749]

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The cytochrome (cyt) b(6)f complex and Stt7 kinase regulate the antenna sizes of photosystems I and II through state transitions, which are mediated by a reversible phosphorylation of light harvesting complexes II, depending on the redox state of the plastoquinone pool. When the pool is reduced, the cyt b(6)f activates the Stt7 kinase through a mechanism that is still poorly understood. After random mutagenesis of the chloroplast petD gene, coding for subunit IV of the cyt b(6)f complex, and complementation of a Delta petD host strain by chloroplast transformation, we screened for impaired state transitions in vivo by chlorophyll fluorescence imaging. We show that residues Asn122, Tyr124, and Arg125 in the stromal loop linking helices F and G of cyt b(6)f subunit IV are crucial for state transitions. In vitro reconstitution experiments with purified cyt b(6)f and recombinant Stt7 kinase domain show that cyt b(6)f enhances Stt7 autophosphorylation and that the Arg125 residue is directly involved in this process. The peripheral stromal structure of the cyt b(6)f complex had, until now, no reported function. Evidence is now provided of a direct interaction with Stt7 on the stromal side of the membrane.

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