DNA translocation through solid-state nanopore

Solid-state nanopore is a promising alternative for current nucleic acid analysis in DNA sequencing. In this work, we employed current-stimulus dielectric breakdown to fabricate a 2 nm nanopore on SiNx membrane. The effect of this 2 nm SiNx nanopore was then examined by comparing the translocation behaviors of four types of ssDNA through this nanopore. Current signal collected from the nanopore can distinguish the four types of ssDNA with 30 nucleobases and 100 nucleobases, providinginformation about the sequence and structure of the ssDNA molecule.