期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 28, 期 5, 页码 784-795出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1712.12014
关键词
Bacillus amyloliquefaciens; lipopeptides; metabolomics; metabolic flux
资金
- National Key Basic Research Program of China [2015CB755903]
- COMRA program [DY135-B-01]
- Yantai Marine Economic Innovation and Development Demonstration City Industrial Chain Collaborative Innovation Project [YHCX-SW-L-201703]
- China Polar Environment Comprehensive Investigation and Assessment Program [CHINARE2017-04-03, CHINARE2017-01-05]
- Xiamen Ocean Economic Innovation and Development Demonstration Project [16PZP001SF16]
Bacillus amyloliquefaciens Pc3 was isolated from Antarctic seawater with antifungal activity. In order to investigate the metabolic regulation mechanism in the biosynthesis of lipopeptides in B. amyloliquefaciens Pc3, GC/MS-based metabolomics was used when exogenous indole was added. The intracellular metabolite profiles showed decreased asparagine, aspartic acid, glutamine, glutamic acid, threonine, valine, isoleucine, hexadecanoic acid, and octadecanoic acid in the indole-treated groups, which were involved in the biosynthesis of lipopeptides. B. amyloliquefaciens Pc3 exhibited a growth promotion, bacterial total protein increase, and lipopeptide biosynthesis inhibition upon the addition of indole. Besides this, real-time PCR analysis further revealed that the transcription of lipopeptide biosynthesis genes ituD, fenA, and srfA-A were downregulated by indole with 22.4-, 21.98-, and 26.0-fold, respectively. It therefore was speculated that as the metabolic flux of most of the amino acids and fatty acids were transferred to the synthesis of proteins and biomass, lipopeptide biosynthesis was weakened owing to the lack of precursor amino acids and fatty acids.
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