期刊
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
卷 47, 期 8, 页码 782-788出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2017.1342260
关键词
Aspergillus oryzae: biodegradation pathway glyphosate; intermediate metabolites
类别
资金
- National Natural Science Foundation of P. R. China [31160336]
- Analysis and Test Center of Nanchang University [2012039]
Aspergillus oryzae A-F02, a glyphosate-degrading fungus, was isolated from an aeration tank in a pesticide factory. The pathway and rate-limiting step of glyphosate (GP) degradation were investigated through metabolite analysis. GP, aminomethylphosphonic acid (AMPA), and methylamine were detected in the fermentation liquid of A. oryzae A-F02, whereas sarcosine and glycine were not. The pathway of GP degradation in A. oryzae A-F02 was revealed: GP was first degraded into AMPA, which was then degraded into methylamine. Finally, methylamine was further degraded into other products. Investigating the effects of the exogenous addition of substrates and metabolites showed that the degradation of GP to AMPA is the rate-limiting step of GP degradation by A. oryzae A-F02. In addition, the accumulation of AMPA and methylamine did not cause feedback inhibition in GP degradation. Results showed that degrading GP to AMPA was a crucial step in the degradation of GP, which determines the degradation rate of GP by A. oryzae A-F02.
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