期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 28, 期 4, 页码 638-644出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1710.10037
关键词
Immobilization; encapsulation; protein-inorganic hybrid; reusability; Thermomyces lanuginosus; xylanase
资金
- Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Ministry of Trade, Industry & Energy, Republic of Korea [20153030091450]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [NRF-2013R1A1A2012159, NRF-2013R1A1A2007561]
- KU Research Professor Program of Konkuk University
- KU Brain Pool Fellowship of Konkuk University
- National Research Foundation of Korea [2015R1D1A1A01061227, 22A20130012144] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at 4 degrees C for 72 h, using 0.25 mg/ml protein, and efficient immobilization of xylanase was observed, with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. Immobilized xylanase showed high residual activity at broad pH and temperature ranges. Using birchwood xylan as a substrate, the V-max and K-m values of xylanase nanoflowers were 1.60 mg/ml and 455 mu mol/min/mg protein, compared with 1.42 mg/ml and 300 mu mol/min/mg protein, respectively, for the free enzyme. After 5 and 10 cycles of reuse, the xylanase nanoflowers retained 87.5% and 75.8% residual activity, respectively. These results demonstrate that xylanase immobilization using a protein-inorganic hybrid nanoflower system is an effective approach for its potential biotechnological applications.
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