期刊
POLYMER CHEMISTRY
卷 8, 期 2, 页码 451-460出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6py01787h
关键词
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资金
- European Research Council [307701]
- Austrian Science Fund (FWF) [P27555]
- Universite de Geneve
- Austrian Science Fund (FWF) [P27555] Funding Source: Austrian Science Fund (FWF)
- European Research Council (ERC) [307701] Funding Source: European Research Council (ERC)
- Austrian Science Fund (FWF) [P 27555] Funding Source: researchfish
The possibility of the direct encapsulation of living cells via two-photon induced photopolymerization enables the microfabrication of hydrogel scaffolds with high initial cell loadings and intimate matrix-cell contact. While highly efficient water-soluble two-photon initiators based on benzylidene ketone dyes have been developed, they exhibit considerable cyto- and phototoxicity. To address the problem of photoinitiator migration from the extracellular matrix into the cytosol, a two-photon initiator bound to a polymeric hyaluronan backbone (HAPI) was synthesized in this work. HAPI exhibited a distinct improvement of cytocompatibility compared to a reference two-photon initiator. Basic photophysical investigations were performed to characterize the absorption and fluorescence behavior of HAPI. Laser scanning microscopy was used to visualize and confirm the hindered transmembrane migration behavior of HAPI. The performance of HAPI was tested in two-photon polymerization at exceedingly high printing speeds of 100 mm s(-1) producing gelatin-based complex 3D hydrogel scaffolds with a water content of 85%. The photodamage of the structuring process was low and viable MC3T3 cells embedded in the gel were monitored for several days after structuring.
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