期刊
FEMS MICROBIOLOGY ECOLOGY
卷 94, 期 7, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiy089
关键词
subsurface; metagenomics; archaea; methane oxidation
类别
资金
- National Science Foundation [EAR-0948659, EAR-0948335]
- Deep Carbon Observatory (Alfred P. Sloan Foundation) [48045]
- Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant of the Netherlands Ministry of Education, Culture and Science [024.002.002]
- Netherlands Organisation for Scientific Research (NWO)
- Division Of Environmental Biology
- Direct For Biological Sciences [1441646] Funding Source: National Science Foundation
The concentrations of electron donors and acceptors in the terrestrial subsurface biosphere fluctuate due to migration and mixing of subsurface fluids, but the mechanisms and rates at which microbial communities respond to these changes are largely unknown. Subsurface microbial communities exhibit long cellular turnover times and are often considered relatively static-generating just enough ATP for cellular maintenance. Here, we investigated how subsurface populations of CH4 oxidizers respond to changes in electron acceptor availability by monitoring the biological and geochemical composition in a 1339 m-below-land-surface (mbls) fluid-filled fracture over the course of both longer (2.5 year) and shorter (2-week) time scales. Using a combination of metagenomic, metatranscriptomic, and metaproteomic analyses, we observe that the CH4 oxidizers within the subsurface microbial community change in coordination with electron acceptor availability over time. We then validate these findings through a series of C-13-CH4 laboratory incubation experiments, highlighting a connection between composition of subsurface CH4 oxidizing communities and electron acceptor availability.
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