4.6 Article

Thallium stimulates ethanol production in immortalized hippocampal neurons

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PLOS ONE
卷 12, 期 11, 页码 -

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PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0188351

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Lactate and ethanol (EtOH) were determined in cell culture medium (CCM) of immortalized hippocampal neurons (HN9.10e cell line) before and after incubation with Thallium (Tl). This cell line is a reliable, in vitro model of one of the most vulnerable regions of central nervous system. Cells were incubated for 48 h with three different single Tl doses: 1, 10, 100 mu g/L (corresponding to 4.9, 49 and 490 nM, respectively). After 48 h, neurons were reperfused with fresh CCM every 24/48 h until 7 days after the treatment and the removed CCM was collected and analysed. Confocal microscopy was employed to observe morphological changes. EtOH was determined by head space-solid phase microextraction -gas chromatography -mass spectrometry (HS-SPME-GCMS), lactate by RP-HPLC with UV detection. Tl exposure had significant effects on neuronal growth rate and morphology. The damage degree was dose-dependent. In not exposed cells, EtOH concentration was 0.18 +/- 0.013 mM, which represents about 5% of lactate concentration (3.4 +/- 0.10 mM). After Tl exposure lactate and EtOH increased. In CCM of 100 and 10 mu g/L Tl-treated cells, lactate increased 24 h after reperfusion up to 2 and 3.3 times the control value, respectively. In CCM of 10 and 100 mu g/L Tl-treated cells 24 h after reperfusion, EtOH increased up to 0.3 and 0.58 mmol/L. respectively. These results are consistent with significant alterations in energy metabolism, despite the low doses of Tl employed and the relatively short incubation time.

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