期刊
FEMS MICROBIOLOGY ECOLOGY
卷 94, 期 7, 页码 -出版社
OXFORD UNIV PRESS
DOI: 10.1093/femsec/fiy095
关键词
anaerobic digestion; psychrophilic; hydrolysis; microbial community structure; adaptation
类别
资金
- Science Foundation Ireland, through a Charles Parsons Award [06_CP_E006]
- Science Foundation Ireland, through Investigator Programme Grant [14/IA/2371]
- Irish Environmental Protection Agency [2014-W-LS-7]
- Science Foundation Ireland Starting Investigator-COFUND fellowship [11/SIRG/B2159]
- NERC fellowship [NE/L011956/1]
- NERC [NE/L011956/1] Funding Source: UKRI
- Science Foundation Ireland (SFI) [11/SIRG/B2159] Funding Source: Science Foundation Ireland (SFI)
- Environmental Protection Agency Ireland (EPA) [2014-W-LS-7] Funding Source: Environmental Protection Agency Ireland (EPA)
The development and activity of a cold-adapting microbial community was monitored during low-temperature anaerobic digestion (LtAD) treatment of wastewater. Two replicate hybrid anaerobic sludge bed-fixed-film reactors treated a synthetic sewage wastewater at 12 degrees C, at organic loading rates of 0.25-1.0 kg chemical oxygen demand (COD) m(-3) d(-1), over 889 days. The inoculum was obtained from a full-scale anaerobic digestion reactor, which was operated at 37 degrees C. Both LtAD reactors readily degraded the influent with COD removal efficiencies regularly exceeding 78% for both the total and soluble COD fractions. The biomass from both reactors was sampled temporally and tested for activity against hydrolytic and methanogenic substrates at 12 degrees C and 37 degrees C. Data indicated that significantly enhanced low-temperature hydrolytic and methanogenic activity developed in both systems. For example, the hydrolysis rate constant (k) at 12 degrees C had increased 20-30-fold by comparison to the inoculum by day 500. Substrate affinity also increased for hydrolytic substrates at low temperature. Next generation sequencing demonstrated that a shift in a community structure occurred over the trial, involving a 1-log-fold change in 25 SEQS (OTU-free approach) from the inoculum. Microbial community structure changes and process performance were replicable in the LtAD reactors.
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