期刊
PLOS ONE
卷 12, 期 11, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0187544
关键词
-
资金
- Research Council of Norway through the MarPol project [221576]
Chitin, a polymer of beta(1-4)-linked N-acetylglucosamine found in e.g. arthropods, is a valuable resource that may be used to produce chitosan and chitooligosaccharides, two compounds with considerable industrial and biomedical potential. Deacetylating enzymes may be used to tailor the properties of chitin and its derived products. Here, we describe a novel CE4 enzyme originating from a marine Arthrobacterspecies (ArCE4A). Crystal structures of this novel deacetylase were determined, with and without bound chitobiose [(GIcNAc)(2)], and refined to 2.1 angstrom and 1.6 angstrom, respectively. In-depth biochemical characterization showed that ArCE4A has broad substrate specificity, with higher activity against longer oligosaccharides. Mass spectrometry-based sequencing of reaction products generated from a fully acetylated pentamer showed that internal sugars are more prone to deacetylation than the ends. These enzyme properties are discussed in the light of the structure of the enzymeligand complex, which adds valuable information to our still rather limited knowledge on enzyme-substrate interactions in the CE4 family.
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