期刊
PLANT SCIENCE
卷 256, 期 -, 页码 160-169出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2016.12.007
关键词
Saussurea involucrata Kar. et Kir.; SiDHN; Cold resistance; Drought resistance; Tobacco
资金
- National Natural Sciences Foundation of China [31160049, 31301058]
Saussurea involucrata Kar. et Kir. is a hardy, dicotyledonous plant featured by strong tolerance to severe stress conditions. In a previous study, we created a cDNA library to characterize genetic factors associated with the cold acclimation response of S. involucrata. The full-length cDNA of one dehydrin-like gene, SiDHN, was obtained by RT-PCR and the SiDHN gene was further characterized in this study. The full-length SiDHN cDNA consists of 703 base pairs (bp) with a 333 bp open reading frame encoding a protein comprising 111 amino acid residues. The alignment of the deduced amino acid sequence showed that SiDHN protein shared 36% identity with one homolog in Helianthus annuus. To evaluate its biological functions, 35S:SiDHN recombinant plasmid was introduced into tobacco using Agrobacterium tumefaciens and the resistance of transgenic plants to freezing and drought stresses were analyzed. Compared with the wild-type, transgenic tobacco plants showed greater resistance to freezing and drought stresses. In this study, we provided evidence that SiDHN can enhance plant cold and drought resistance, suggesting that SiDHN could be potentially used to genetically improve plant tolerance to abiotic stresses. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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